#DOTBOT BANK PROFESSIONAL#

Dot always has the best massage therapists.вЂќ The band was very happy and it was great to have someone show up with such short notice.' вЂќ I like the idea that all Dot Bots are auditioned/tested before they get on the team.вЂќ

#DOTBOT BANK PROFESSIONAL#

Dot team! Your team is consistent, professional and give an outstanding massage. Everyone was VERY happy today so I can't thank you enough'вЂќ

вЂњ'The Dotbots were really really nice Ladies.

We love your team, please send more Dot Bots asap'вЂќ

вЂњ'Both Kaya and Skye did a great job for us.

They are really a pleasure to work with'вЂќД®veryone was happy with their massage - so thumbs up!

вЂњ'you KNOW I would only use your team Dot.

Kristin Schloesser Tour Manager -Allyssa Edwards Scream it lymi tour Get Loud tour Dot aka the Queen helping us out on this run!! Thanks Romana, def will hit you up next time round. She took care of the band-members who all were left amazed and startled by her touch ) Romi (Prague Dotbot) came by, arrived early, was very well prepped (had her own table, scented candles, oils, towels) and had the brightest smile we'd seen in days! Very on point and definitely made for an amazing show at night - thank you again! He provided a table, towels etc which made it very easy to work with.

вЂњLars (Stockholm Dotbot) took care of the band-members and left all in awe!.

The person that came out to our Chicago show (Ali) was amazing, and because of her i decided to look into getting more book for the tour. Your Dotbots are doing a great job for us out here and making happiness for our bands and crew.ДЄlso. Thank you again for taking such good care of us. Of course all the therapists have been great on this run' Jan.

вЂњ'We really like Denise (Boston Dotbot) she does a great job in Mass.

Members of the Structural Biology and Proteomics research group of the Institute of Life Sciences of NUS. 2Д¬urrent address: Burnham Institute for Medical Research, La Jolla, CA 92037. of Chemistry and Biochemistry, University of California at San Diego, La Jolla, CA 92093. The on-line version of this article (available at ) contains supplemental Fig. Section 1734 solely to indicate this fact. This article must therefore be hereby marked вЂњ advertisementвЂќ in accordance with 18 U.S.C. The costs of publication of this article were defrayed in part by the payment of page charges. S.) and Biomedical Research Council Grant 04/1/21/19/315 from the Agency for Science Technology and Research (A*STAR), Singapore (to F. This work was supported by Academic Research Fund, National University of Singapore Grants R-154-000-349-112 (to Y. The atomic coordinates and structure factors (code 3EBK and 3EBW) have been deposited in the Protein Data Bank, Research Collaboratory for Structural Bioinformatics, Rutgers University, New Brunswick, NJ ( ). A major IgE binding epitope unique to Per a 4 is found on the loops at the bottom of the ОІ-barrel that may aid the development of hypoallergens for immunotherapy.

Bla g 4 and Per a 4 are found to be cross-reactive in sera IgE binding, at least in the Singaporean Chinese population tested. Putative ligand binding sites of both allergens are determined by docking of the juvenile hormone III inside the ОІ-barrel and found to interact with the ligand using non-conserved residues. Bla g 4 is found to be a monomer, whereas Per a 4 exists as a dimer in solution with a novel dimeric interface involving residues from loops at the top and bottom of the ОІ-barrel. The C-terminal helix of Bla g 4 is bent and greatly extended toward the N terminus. Both Bla g 4 and Per a 4 contain two long-range disulfide bonds linking the N and C termini to a ОІ-barrel. Here we report for the first time the structures of two major cockroach allergens, Bla g 4 and Per a 4, that adopt a typical lipocalin fold but with distinct structural features as compared with other known lipocalin allergens.

Inhalant allergens from cockroaches are an important cause of asthma to millions of individuals worldwide.

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